Journal:

Article Title: Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1

doi: 10.1046/j.1365-2249.2001.01515.x

Figure Lengend Snippet: a, Western blot analysis showing the position of the scFv-Fc fusion protein. Lane 1, Molecular weight markers (97·4 kD-21·5 kD); Lane 2, III-20-Fc; Lane 3, IV-67-Fc. The fusion proteins were electrophoresed through a 10% SDS-PAG and transferred onto PVDF membrane. The Fc containing proteins were detected with HRP conjugated anti-human IgG and developed using chemiluminescence. b, Silver stained SDS-PAG showing the position of the scFv fusion protein as determined after the western blot analysis in Fig. 4(a). Lane 1, III-20-Fc; Lane 2, IV-67-Fc; Lane 3, Molecular weight markers. The samples were electrophoresed through a 10% SDS-PAG. The gel was stained with silver nitrate to detect proteins.

Article Snippet: The blots were blocked with 3% MPBS and incubated with 9E10 followed by anti-mouse IgG-HRP conjugate and developed using the BioRad chemiluminescence kit (Bio-Rad Laboratories).

Techniques: Western Blot, Molecular Weight, Staining

Journal:

Article Title: Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1

doi: 10.1046/j.1365-2249.2001.01515.x

Figure Lengend Snippet: Dot blot analysis showing the specific binding of fusion proteins to GPRV and PV11·1µg GPRV, PV11 and BSA were dotted onto PVDF membrane. These were detected with III-20-Fc (lane 1), IV-67-Fc (lane 2), Fc (lane 3, negative control) and HRIG (lane 4, positive control) followed by HRP conjugated anti-human IgG. The blots were developed using chemiluminescence.

Article Snippet: The blots were blocked with 3% MPBS and incubated with 9E10 followed by anti-mouse IgG-HRP conjugate and developed using the BioRad chemiluminescence kit (Bio-Rad Laboratories).

Techniques: Dot Blot, Binding Assay, Negative Control, Positive Control